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KMID : 1123920030170061514
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 6 p.1514 ~ p.1518
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Modulation of Aqueous Extracted Angelicae sinensis Radix on Nitric Oxide Production and Pro-inflammatory Cytokine Gene Expressions in RAW 264.7 Macrophage Cells
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Lee Young-Sun
Han Ok-Kyung
Shin Sang-Woo
Park Jong-Hyun
Kwon Young-Kyu
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Abstract
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Angelica sinensis radix, Danggui, is a traditional oriental medication, which has been used to modulate immune response. We report here that aqueous extract of Angelica sinensis radix (ASR) can induces NO production, and inhibit LPS-induced NO production in dose-dependent manner in RAW 264.7 macrophage cells. ASR also induces iNOS mRNA and iNOS protein expression, and exhibit inhibitory effect on iNOS mRNA and protein expression in a dose-dependent manner in LPS-stimulated RAW 264.7 macrophage cells. Cytokines involved in the regulation of inflammatory reaction and immune response may play a role in the pathogenesis. ASR induces. pro-inflammatory cytokine gene expression (IL-1α, IL-1β and IL-6 gene) in a dose-dependent manner, and inhibits the expressions of these cytokines in LPS-stimulated RAW 264.7 macrophage cells. These data indicate that (1) ASR may be a potential therapeutic modulator of NO synthesis in various pathological conditions, and (2) the immunomodulatory effects of ASR may be, in part, associated with the inducing or suppression of pro-inflammatory cytokine gene expressions.
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KEYWORD
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Angelica sinensis radix, NO, iNOS, cytokine, RAW 264.7 cells
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